Affinity labeling by Nathan P. Kaplan, Nathan P. Colowick, William B. Jakoby, PDF

By Nathan P. Kaplan, Nathan P. Colowick, William B. Jakoby, Meir Wilchek

ISBN-10: 0121819469

ISBN-13: 9780121819460

The significantly acclaimed laboratory average, Methods in Enzymology, is among the such a lot hugely revered guides within the box of biochemistry. for the reason that 1955, each one quantity has been eagerly awaited, often consulted, and praised by way of researchers and reviewers alike. The sequence includes a lot fabric nonetheless suitable at the present time - actually an important booklet for researchers in all fields of existence sciences

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G. Wood, J. Biol. Chem. 244, 5810 (1969). s G. H. Reed and S. D. Morgan, Biochemistry 13, 3537 (1974). • K. D. Collins and G. R. Stark, J. Biol. Chem. 246, 6599 (1971). h W. B. Dempsey and E. E. Snell, Biochemistry 2, 1414 (1973). G. E. Lienhard and I. I. Secemski, J. Biol. Chem. 246, 1121 (1973). i E. J. Milner-White and D. C. Watts, Biochem. J. 122, 727 (1971). k D. H. Buttlaire and M. Cohn, J. Biol. Chem. 249, 5733 (1974). K. A. Koetfler and G. P. Hess, Biochemistry 18, 5345 (1974). K. A. Koehler and G.

Wolfenden, Biochemistry 9, 3404 (1970). / / J . M. Chirgwin and E. A. Noltmann, Fed. Proc. 32, 667 (1973). gg D. Cassio, F. LeMoine, J. P. Waller, E. Sandrin, and R. A. Boissonas, Biochemistry 6, 827 (1967). hAW. B. Rowe, R. A. Ronzio, and A. Meister, Biochemistry 8, 2674 (1969). [2] TRANSITION STATE ANALOGS 25 active-site sulfhydryl group of this enzyme, thought to serve as a point of attachment for the developing carboxylic acid in the normal reaction catalyzed by this enzyme, is also a possible site of attachment of the inhibitor, as shown below.

2] 19 TRANSITION STATE ANALOGS such studies make clear the state of the bound analog. This important information can in principle be obtained from the results of spectroscopic studies of enzyme-inhibitor complexes. In the above example, the dishionic form of the bound inhibitor could involve any of the species shown below. Nuclear magnetic resonance (NMR) studies of bound ligands suggest that bound 2-phosphoglycolate is present as the trianion, the enzyme itself taking up a proton as the transition state analog is bound.

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Affinity labeling by Nathan P. Kaplan, Nathan P. Colowick, William B. Jakoby, Meir Wilchek


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